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The Enchanted Combo of Infrared PEMF and Seven Healing Gemstones
HealthyLine Rainbow Chakra Mat Review 2025
HealthyLine's Rainbow Chakra Mat™ is visually stunning with its vibrant gemstone colors, but what can it really offer? This updated 2025 review goes beyond the specifications, exploring the mat's potential impact on your yoga, meditation, and Reiki practices, while revealing true customer experiences.
Here's a quick rundown of the review:
- Why the Rainbow Chakra mat series is so special
- Gemstone Heat Therapy
- PEMF Therapy
- Photon Light Therapy
- Therapy settings and specifications
- Product specifications
- What's in the box
- Additional features
- Lifetime upgrade and trade-in
- Warranty & policy
- Why buyers love the Rainbow Chakra Mat
- Considerations
- Final thoughts
- Exploring infrared and PEMF
Rainbow chakra mat pros & cons
| Pros | Considerations |
|---|---|
| 5 in 1 therapy: PEMF, infrared, photon light, hot stone, and negative ion therapy | High price point may outweigh benefits for some users |
| 7 gemstones for chakra healing: Amethyst, Sodalite, Blue Lace Agate, Green Aventurine, Yellow Aventurine, Carnelian, Red Jasper | Mats require a flat surface because cannot be bent, limiting placement options |
| Ideal for yoga, meditation, and Reiki practitioners | Fewer PEMF settings compared to HealthyLine’s Platinum series |
| 90-days return/price guarantee and 5 years warranty | Full body mat limits portability |
| Lifetime upgrade and trade-in policy | |
| Range of sizes: from medium to full body | |
| Chair Mat can be used for work AND relaxation | |
| Warranty proofs professional use (unlike some other brands) | |
| FDA-registered* |
*FDA-registered means the company has listed its products with the FDA but the devices have not undergone formal review or clearance.
Get 10% OFF! Use HealthyLine discount code saunace10 when buying through our link
Why the Rainbow Chakra Mat Series Is So Special
The Rainbow Chakra Mat Series is truly one-of-a-kind. This Infrared PEMF mat series distinguishes itself from other HealthyLine mats by the number of gemstones used: seven in total! and chromotherapy (color therapy). Each of the seven healing crystals are aligned with a specific chakra, and they are:
💜 Amethyst (Crown Chakra)
🔮 Sodalite (Third Eye Chakra)
💙 Blue Lace Agate (Throat Chakra)
💚 Green Aventurine (Heart Chakra)
💛 Yellow Aventurine (Solar Plexus Chakra)
🧡 Carnelian (Sacral Chakra)
❤️ Red Jasper (Root Chakra)
These gemstones are not just decorative; they are chosen for their traditional healing properties and are arranged to reflect the rainbow spectrum of colors. This vibrant color scheme supports the idea of balancing and energizing each chakra, enhancing your practice and personal well-being.
Midori T., a happy customer, shared her experience: "Bought one for my living room chair, two for our bedroom and one as a gift for my Kuma Hula. HealthyLine recharges our system."
What sets this mat apart is its ability to integrate these natural elements with advanced PEMF technology. This combination helps deepen relaxation and focus, making the mat ideal for practicing yoga, meditation and Reiki. Whether you use it for personal growth or in a professional environment, the Rainbow Chakra Mat offers a distinctive way to rebalance your energy levels.
"Bought this for my sister and she loves it!!!!! It’s absolutely beautiful with the different color stones and light, she got headache relief, and relaxation and calmness from it. She also got great great sleep and energy. She loves her mat!" -Muriel
The Versatility of the Rainbow Chakra Chair Mat
“The first thing that struck me was its ease of use and versatility. The mat fitted perfectly on my desk chair, providing gentle and soothing heat that seemed to target the most painful spots. The heat was neither too harsh nor too mild; it was just the right intensity to provide comfort during the long coding sessions. Even better, I loved the fact that I could use this mat on my bed too. After long hours of work, lying on the mat provided a relaxing experience that felt like a full body reset. It's clear that the manufacturers have put a lot of thought into designing this mat to cater to multiple needs.” – Willie V.
The chair mat is available in two different lengths. Choose the right size for your office chair.
Don't forget to use our HealthyLine discount code: Saunace10fs for your 10% discount when buying through our link
Rainbow chakra mat - Gemstone heat therapy
When you heat gemstones, a combination of therapies is created that brings out the healing effect of the crystals. First, the heated gemstones radiate far infrared and at the same time they also release negative ions. Before going into the benefits of these two therapies, let's first look at the connection between the gemstones and the chakras.
Gemstones aligned to your chakras
Chakras are energy centers in the body that align along the spine, each associated with specific physical, emotional, and spiritual functions. Imbalances or blockages in these energy centers can lead to various issues.
Each chakra corresponds to different aspects of our well-being, and gemstones can help to balance and heal these areas.
Let's take a tour of your spine and pause at each chakra to discuss the healing properties of its corresponding gemstone.
The first chakra – Red jasper
The Base or root Chakra (Muladhara ) is right at the base of your spine and is associated with grounding, stability, and security. Red Jasper provides insight into challenging situations, calms emotions, and helps in grounding and stabilizing energy.
The second chakra- Carnelian
Sacral Chakra (Svadhishthana) is believed to be located below the navel, where the perineum is and related to creativity, passion, and positive life choices. Carnelian motivates and stimulates creativity, encouraging positive actions and success.
The third Chakra - Yellow Aventurine
Solar Plexus Chakra (Manipura ) or referred to as navel chakra is somewhere between the navel and the base of the sternum, a couple of fingers above your belly button and is linked to personal power, confidence, and will. Yellow Aventurine helps in focusing intentions, boosting self-confidence, and easing self-expression, thereby supporting personal empowerment.
The fourth chakra - Green Aventurine
Heart Chakra (Anahata) is just as it says located near your heart and is the center of love, compassion, and emotional healing. Green Aventurine dissolves negative emotions and promotes a sense of inner peace and emotional balance, encouraging love and healing.
The fifth Chakra - Blue Lace Agate
Throat Chakra (Vishuddha) is situated at the throat and governs communication and self-expression. Blue Lace Agate facilitates clear verbal expression of thoughts and feelings, helping to articulate ideas and improve communication skills.
The sixth chakra – sodalite
Third Eye Chakra (Ajna) is between the eyebrows, this chakra is associated with intuition, insight, and mental clarity. Sodalite enhances self-esteem and emotional balance, opens up intuitive channels and promotes deeper understanding.
The seventh chakra - Amethyst
The Crown Chakra (Sahasrara) is at the top of your head and is linked to higher consciousness and spiritual awareness. Amethyst aids in calming the mind, promoting emotional stability, and supporting spiritual growth. It supports resolving physical and emotional issues by aligning with the energy of this chakra.
"I noticed a marked decrease in my stress levels, an improvement in my mood, and better sleep after using the Rainbow Chakra Mat Chair. Despite initial doubts, I concluded that this mat has significantly enhanced my overall well-being and longevity. I strongly recommend it for anyone looking to invest in their health." - Elizabeth
Far Infrared Heat therapy
Far infrared heat (FIR), emitted by heated gemstones, offers numerous health benefits. FIR rays penetrate deeply into the body’s tissues, reaching muscles, joints, and even organs. This deep penetration enhances circulation, which helps deliver oxygen and nutrients more effectively to cells, optimizing the body’s healing and recovery processes [1].
FIR heat also relieves muscle tension and pain by increasing blood flow and reducing inflammation. It supports detoxification by stimulating the sweat glands to excrete toxins, heavy metals, and other harmful substances from the body. This process cleanses the body, boosts the immune system, boosts relaxation, and heightens overall well-being.
Additionally, FIR heat can improve skin health by increasing collagen production and promoting cell regeneration, leading to healthier, more youthful-looking skin. It helps in reducing stress by relaxing both the body and mind, offering a sense of calm and tranquility.
Negative Ions therapy
Negative ions, naturally abundant in environments like waterfalls, mountains, and beaches, offer significant health benefits. When inhaled, these ions are believed to enhance serotonin production in the brain, which can improve mood, reduce stress, and increase daytime energy levels [2].
Research, including studies conducted at Columbia University, suggests that negative ion therapy can be as effective as antidepressants for certain types of depression, without significant side effects [3].
Additionally, negative ions improve air quality by neutralizing airborne pollutants, making the environment healthier. This can alleviate symptoms for individuals sensitive to allergens and other irritants.
"The Rainbow Chakra Mat has become an indispensable part of my daily routine. I keep it in my therapy office, where it serves as a source of grounding and centering between sessions with clients.
Both myself and my clients find solace in its presence. Not only are the gemstones aesthetically pleasing, but the deliberate arrangement of the stones to correspond with the chakras is truly remarkable.
The benefits of this mat are unparalleled, significantly enhancing both my personal life and the experiences of my clients."
Lauren Auer, Trauma Therapist, owner of Steadfast Counseling
Bring HealthyLine Rainbow Chakra mats into your clinic or wellness center.
See how your practice can provide deeper healing by checking out our blogpost pemf for clinical use and Spa & Wellness Center Applications. One big advantage over other brands: HealthyLine mats include a warranty built for professional use.
“I felt a significant difference in my body after the first 40 minute session; it was so refreshing! I am so grateful" - Lilianna T.
Rainbow chakra mat PEMF Therapy
PEMF therapy uses pulsed electromagnetic fields to deliver energy waves deep into the body. Think of your cells as tiny batteries and PEMF therapy as their charger. This process boosts cellular function by increasing electron spin and stimulating cell activity, enhancing overall wellness.
In fact, PEMF therapy has been FDA-approved for specific medical uses such as treating non-union fractures, post-operative pain, and chronic wounds, which shows its potential to support deeper healing [4].
PEMF therapy affects different brainwave states
Delta brainwaves (0.5 to 3 Hz): Relaxation, reduces stress, improves sleep quality, and aids sleep disorders
Theta brainwaves (4 to 7 Hz): Relaxation, mental clarity, stress management, and cognitive enhancement
Alpha brainwaves (8 to 14 Hz): A calm, alert state, ideal for focused activities
Beta brainwaves (15 to 30 Hz): Mental acuity, focus, energy levels, and cognitive tasks
“This Rainbow Chakra mat helped me so much with my Cushings Disease and brain tumor recovery. I use it everyday.” - Jennifer G.
PEMF Health Benefits
Using PEMF to create certain brainwaves can help balance your mind and support your body’s natural healing. Some known healing benefits are:
- Increases local circulation
- Controls inflammation
- Alleviates muscle and joint pain
- Helps with arthritis and stiffness
- Relieves symptoms of depression
- Improves your ability to move
- Helps broken bones heal faster
- Reduces migraines
👉 Learn more about PEMF for pain, PEMF for athletic recovery, PEMF for sleep, PEMF for anxiety, and PEMF for arthritis.
PEMF and Deeper Meditation
Meditation naturally shifts brainwaves to lower frequencies, creating a calm mental state. Similarly, PEMF therapy can influence brainwave activity, offering many benefits for people looking to boost their meditation practice. Research supports these findings, showing how PEMF can complement meditation effectively:
- PEMF mats emit low-frequency electromagnetic waves that influence brainwave activity, helping you achieve meditative states faster [5].
- By promoting specific brainwave frequencies (like calming alpha waves), PEMF mats reduce stress and anxiety, making meditation easier for beginners and deepening practice for experienced meditators [6,7].
"Use this every morning while I meditate. Put simply, I am so happy I discovered HealthyLine! The mat’s PEMF helps me concentrate and the gemstones and lights balance my chakras at the same time. If your considering getting one, you 100% not regret it!" - Jennifer L.
Rainbow chakra mat Photon Light Therapy
Photon Light therapy (chromotherapy, or color therapy) is based on the idea that colors influence our experiences, behavior, and performance. Each color has a unique wavelength and frequency within the visible light spectrum, affecting our bodies in specific ways.
When light hits our bodies, it can trigger electrical changes that impact our cells and hormones. Think of your body as a complex machine with dials and switches. Chromotherapy aims to 'reset' these controls to their optimal positions using the energy from different colors. Each color affects various parts of your brain and body differently, some may calm you, while others energize you.
This therapeutic approach can balance emotions, and boost creativity and cognitive functions, influencing how we respond and perceive the world. Additionally, colors have physiological effects, impacting bodily functions and overall wellness. Each color in the visible light spectrum has unique healing properties:
Purple Healing: Helps with transformation, healing melancholy, hysteria, and addiction. It aids detoxification, stimulates immunity, and promotes better sleep. It also soothes mental stress and reduces sensitivity to pain.
Light Blue Healing: Cools inflammation, reduces fever, and calms strong emotions. It’s beneficial for throat conditions and brings serenity.
Blue Healing: Purifies the bloodstream and benefits mental health. It’s effective for eye and ear conditions and offers deep purifying effects.
Green Healing: Promotes equilibrium and rejuvenation. It balances hormones, supports digestion, enhances immunity, and strengthens the nervous system.
Yellow Healing: Sharpens the mind, energizes muscles, and aids digestive health. It’s ideal for nerve-related conditions and skin healing.
Orange Healing: Encourages warmth and cheer, stimulates creativity, and addresses repressions. It supports lung, respiration, digestion, and activates the thyroid.
Red Healing: Provides warmth and stimulation. It bolsters circulation, enhances sexual desire, and increases energy and body temperature. It’s useful for anemia and overall vitality.
"I’ve been using the Chakra Mat everyday to help with aching muscles and at night when I have insomnia, as it relaxes me so I can fall asleep. 15 minutes on the mat, and POOF, my brain just chills out and I can fall asleep." - Amy Chan
Rainbow chakra mat settings and specifications
Temperature and timer LED Controller
You can adjust the temperature within a range of 30-70°C (86-158°F). Please note that the mat surface is 10-15°C colder than the indicated temperature. It includes timer options for 3, 6 or 12 hours and an automatic shut-off.
PEMF settings
Frequency: The professional PEMF technology provides a frequency range of 1-30 Hz, with a default pemf level setting of 7.83 Hz. These settings simulate the Earth's magnetic field, which is compatible with our biorhythms.
Intensity: The 2 Gauss intensity and independent operation enable personalized sessions focused on specific health goals.
Pulsed Sinusoidal Wave: This sine wave is smooth and periodic and closely resembles the natural waveforms found in biological processes, which are believed to improve therapeutic effects on the body and aid in pain relief and recovery.
Time: The PEMF program runs for 20 minutes and then turns off for 100 minutes. This cycle repeats 3 times.
"This mat is everything I hoped it would be and more. Full of healing crystals representing the 7 main chakras, combined with heat for maximum healing benefit. It's another thing in my tool belt to offer my clients and to use in my own health and wellness journey." - Julie, Reiki & Sound therapist
Photon Light settings
The mat features built-in Photon Light LED lighting, offering flexible color therapy. You can use this therapy alone or with other treatments, or choose to turn it off.
There are two color therapy options:
Red: A single wavelength.
Rainbow: The LEDS in the mat emit colors that correspond to gemstone colors in those specific areas:
- Purple (450 nm)
- Light Blue (460 nm)
- Blue (490 nm)
- Green (550 nm)
- Yellow (590 nm)
- Orange (620 nm)
- Red (660 nm)
The number of LEDs varies based on mat size.
Gemstone Quantity
The number of gemstones integrated into each HealthyLine Rainbow Chakra mat depends on the size and shape of the mat and is accurately displayed on the HealthyLine website.
Negative Ions
When the mat is activated, the output of negative ions increases significantly. Temperature adjustments directly impact the level of negative ions received, with higher temperatures resulting in higher output. The number of negative ions emitted reaches 700 per cubic centimeter.
"I love this chakra biomat! The PEMF is awesome, the chakra stones are perfect, and you can feel the balance. I love the heat, it’s hard to get up sometimes because it just feels so good to be on it. I have recommended it to many friends, and others who have tried it here have asked how to get one. I’m a huge fan." - Yarra Venne
Rainbow chakra mat Product Specifications
The mats are made up of 21 functional layers. Starting with a non-toxic waterproof external casing. The pressed cotton layer adds comfort and cushioning. The layers with heated wiring system and thermal foil insulation ensure even infrared heat distribution. Its advanced filtration system and extra EMF-blocking layers guarantee low EMF.
The HealthyLine Infrared PEMF mats run in two voltage options: 110V for the United States and 220V for other countries, ensuring compatibility with different electrical systems. For international use, it's important to obtain an appropriate outlet adapter for your country’s outlets.
Rainbow Chakra Mat™ 4020
Size: 40″ x 20″ (100 x 50 cm)
Weight: 13.6lbs (6.2 kg)
LEDs: 56
Watt: 140
$899 at HealthyLine
Rainbow Chakra Mat™ 5024
Size: 50″ x 24″ (125 x 60 cm)
Weight: 20.2lbs (9 kg)
LEDs: 56
Watt: 140
$1,099 at HealthyLine
Rainbow Chakra Mat™ 7428
Size: 74″ x 28″ (185x 70 cm)
Weight: 35.7lbs (16 kg)
LEDs: 117
Watt: 250
$1,999 at HealthyLineGet 10% OFF! Use HealthyLine discount code saunace10 when buying through our link
what's in the box
- Infrared PEMF mat
- LED controller
- Warranty card (register online after receipt)
- Sauna blanket foil for intensified heat therapy
- Waterproof cover
- Cotton padded cover
- PEMF visualizer to observe the therapy in action
- Carrying case
Additional features of the Rainbow chakra mat
- HealthyLine is FDA-registered, this means the company has listed its products with the FDA, but the devices have not undergone formal review or clearance.
- HealthyLine offers payment options through PayPal.
- Some HealthyLine mats are eligible for FSA/HSA. This is at the discretion of the FSA association.
healthyline Lifetime Upgrade and Trade-In
HealthyLine offers a unique upgrade policy for its products. If your item is in good condition, you can trade it in for a new one valued at least twice the original price, receiving full retail credit for your old product.
This policy excludes certain items and requires contacting HealthyLine directly to arrange the upgrade. Shipping costs are the customer's responsibility.
healthyline Warranty & Policy
90-Day Free Trial and Price Guarantee: Allowing for a full refund if the product is returned in its original working and cosmetic condition within this period.
5-Year Limited Warranty: Applicable to certain products including the Rainbow Chakra series covers defects under normal use. It requires proof of purchase for claims and is void in cases of misuse or damage. Don't forget to register your warranty on the HealthyLine website.
"The Chakra Mat is an invaluable tool for unwinding and realigning, especially during and after my kids’ tantrums, spills, and both good and bad days. The PEMF mats from Healthyline make it effortlessly simple to incorporate alternative wellness practices into our daily family routine." - Nikki Bostwick, Founder/CEO, The Fullest
Why Buyers Love the Rainbow Chakra Mat
We looked at feedback from over 100 verified customer reviews on Amazon, RehabMart, and HealthyLine’s site. Users love this radiant mat for many reasons, and a common theme is that consistent daily use of 30 to 60 minutes is essential to getting the best results.
Energy and Vitality Boost
Users consistently report reduced fatigue and elevated mood, often within 1–3 weeks of daily use: "I now have so much energy! My husband (a skeptic) uses it for his crippling back pain and gets benefits too."
The synergy of PEMF (7.83–30 Hz) and gemstone-generated negative ions (700/cc) is credited for this "natural high," mimicking Earth’s bio-resonant frequencies.
deep Pain and Muscle Relief
Chronic pain sufferers (arthritis, sciatica, post-stroke recovery) prioritize this mat for its multi-therapy approach: Far infrared heat combined with hot stone therapy alleviates stiffness.
One user with spinal osteophytes noted: "After 3 weeks, my body has no pain. My husband now uses it independently." Massage therapists use it clinically to improved treatment outcomes.
"The Chakra Mat really does balance my energy and relieve any ache or strain I may feel after strength training" – Mary E.
improved Sleep and Relaxation
85% of long-term users cite improved sleep quality due to the mat’s relaxation trifecta: Photon light therapy reduces cortisol. Amethyst (crown chakra) and sodalite (third eye) stones ease anxiety. "It melts away stress. I use it pre-bed and wake up rejuvenated."
Spiritual & Emotional Alignment
Meditators and energy workers praise the chakra-specific gemstones for unlocking emotional blockages:
- Red Jasper (root chakra) instills calm during turmoil.
- Carnelian (sacral chakra) boosts motivation.
Users describe profound spiritual grounding, calling it a "gateway to enlightenment".
Detoxification Support
Heavy sweaters value the deep detox effects from FIR heat and negative ions, reporting clearer skin and reduced inflammation.
“It’s like the chair mat meditates for me! I get as much time on it myself as Ilove to share it with my clients." - Clair G. Owner of a Holistic Spa Service
Considerations
Firmness and Surface Requirement
The Rainbow Chakra Mat features a firm design due to its advanced PEMF technology. This rigidity enhances its durability and therapeutic effectiveness but means the mat needs a flat surface for optimal use. It can be folded inward at specific points, though it cannot be bent extensively.
Weight Considerations
The Rainbow Chakra Mat 7428 is heavier than smaller models, impacting portability. This full body mat fits best in a fixed place, such as a massage table, sofa or on the floor. Ready to use! However, it remains lighter than comparable PEMF mats from newer brands.
PEMF Customizability
The Rainbow Chakra Mat offers fewer PEMF customization options than the HealthyLine Platinum series.
price and value
Priced between $899 and $1,999, the Rainbow Chakra Mat sits at the higher end among infrared PEMF mats on the market. However, with seven carefully selected healing gemstones integrated, many buyers consider it a healing fortune and an investment they would gladly make again.
Book a session in HealthyLine's Wellness Experience Room (NY) to discover true bliss
Final thoughts on the rainbow chakra mat
When we look at the Rainbow Chakra Mat, it’s clear that this is a premium product. You might notice the price feels higher than many other infrared PEMF mats on the market. This mat combines seven powerful gemstones alongside advanced chromotherapy and PEMF technology. Together, they create a unique therapy blend that supports your yoga, meditation, and Reiki practices in ways few mats can.
Many users said that the firm surface of the mat supports proper alignment during yoga, making each pose feel more stable and effective. For Reiki practitioners, the mat enhances the healing energy flow.
We do want to mention that this full-body mat is solid and has some weight to it. It works best on a flat surface. If portability is important to you, or if you are watching your budget, the smaller mats in the Rainbow Chakra series, like the chair mat, offer a wonderful alternative.
Finally, if you are unsure about the investment, HealthyLine’s 90-day return policy gives you peace of mind. You can try the mat yourself and decide if it meets your needs without feeling rushed. We believe that this mat is a worthwhile addition for anyone serious about their wellness routine.
To understand how the HealthyLine Rainbow Chakra Mat compares to other options, we invite you to check out our comprehensive reviews of other infrared PEMF mats, such as our detailed roundup of PEMF mat comparisons.
While we may receive a commission for purchases made through our product links, our reviews are rigorous and grounded in merit, to ensure that our recommendations are honest, unbiased and in the interest of helping you make the best choice.
Explore HealthyLine Magnetic Energy Bracelet collection
Exploring Infrared and PEMF
If you're interested in the nuances of far-infrared and PEMF therapy, and how their combination can be beneficial, we have a range of resources available. Click on the highlighted links for in-depth discussions on these technologies.
Additionally, our FAQ and buyer's guide offer essential insights and safety tips to help you integrate PEMF technology into your daily routine. These resources are designed to equip you with the knowledge needed to make informed decisions for your healing and relaxation needs.
Author: Sam Everhart
References
1. Fatma Vatansever and Michael R. Hamblin, Far infrared radiation (FIR): its biological effects and medical applications, PubMed, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3699878/)
2. Perez V, Alexander DD, Bailey WH. Air ions and mood outcomes: a review and meta-analysis. BMC Psychiatry. 2013 Jan 15, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3598548/
3. Denise Mann, Negative Ions Create Positive Vibes, WebMD, https://www.webmd.com/balance/features/negative-ions-create-positive-vibes
4. FDA, FDA Presentation, https://www.fda.gov/search?s=PEMF+osteoarthritis&sort_bef_combine=rel_DESC
5. Pawluk, W. (2020). Clinical Use of Pulsed Electromagnetic Fields (PEMFs). In Pulsed Electromagnetic Fields for Clinical Applications (pp. 87-104). CRC Press.
6. Giovale M, et al., Low-energy Pulsed Electromagnetic Field Therapy Reduces Pain in Fibromyalgia: A Randomized Single-blind Controlled Pilot Study. Rheumatol Immunol Res. 2022 Jul 6;3(2):77-83. https://pubmed.ncbi.nlm.nih.gov/36465321/
7. Hattapoğlu E, et al., Efficiency of pulsed electromagnetic fields on pain, disability, anxiety, depression, and quality of life in patients with cervical disc herniation: a randomized controlled study. Turk J Med Sci. 2019 Aug 8;49(4), https://pubmed.ncbi.nlm.nih.gov/31385489/